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Fragments of DNA and RNA molecules separated by gel electrophoresis are trans...
One ml of TRI Reagent is sufficient to isolate RNA, DNA, and protein from 50-100 mg of tissue, 5-10 ´ 106 cells, or 10 cm2 of culture dish surface for cells grown in monolayer.
This procedure is an improvement of the single-step method reported by Chomczynski and Sacchi for total RNA isolation.
TRI Reagent performs well with large or small amounts of tissue or cells and many samples can be simultaneously extracted.
This is one of the most effective methods for isolating total RNA and can be completed in only 1 hour starting with fresh tissue or cells.
The procedure is very effective for isolating RNA molecules of all types from 0.1-15 kb in length.